FREQUENTLY ASKED QUESTIONS
All of our Real-Time PCR assays are simplex. Different target species assays can be carried out in one single PCR run simultaneously, as their PCR conditions are equal.
To perform diagnostics with our Kit you need a Real-Time PCR equipment. For sample preparation you need standard equipment for a molecular laboratory.
The DNA extraction kit(s) require a thermoincubator and a micro centrifuge (tube format) or plate centrifuge (plate format) which can fit a plate assembly 58 mm high.
Our assays use a fluorescent DNA binding dye for detection and therefore will produce good results with any Real-Time PCR machine that has a FAM/SYBR Green channel for detection.
The ClearDetections PCR mix does not contain any fluorescence reference dye but if required it can be added separately to the reconstituted PCR mix (e.g. Rox or Fluorescein).